Filters
Secondary Antibodies
Secondary antibodies are immunoglobulins that specifically bind to primary antibodies rather than directly binding to target antigens. They recognize the constant region (Fc region) of the primary antibody and are commonly labeled with enzymes or fluorophores for detection purposes. They are widely used in immunoassays such as Western blot, ELISA, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to provide signal amplification and improve detection sensitivity. Because multiple secondary antibodies can bind to a single primary antibody, they significantly enhance assay performance and signal strength. Secondary antibodies are selected based on the host species and isotype of the primary antibody and are available in various conjugated forms such as HRP, AP, and fluorescent dyes for different experimental applications.
HRP-Conjugated Secondary Antibodies
HRP-conjugated secondary antibodies enable chemiluminescent and colorimetric detection in western blot and ELISA workflows through horseradish peroxidase enzymatic amplification.
Fluorophore-Conjugated Secondary Antibodies
Fluorophore-conjugated secondary antibodies are labeled with dyes such as Alexa Fluor or FITC for sensitive multiplex detection in immunofluorescence and flow cytometry.
Anti-Mouse Secondary Antibodies
Anti-mouse secondary antibodies specifically recognize mouse-derived primary antibodies, supporting reliable detection across a wide range of immunoassay applications.
Anti-Rabbit Secondary Antibodies
Anti-rabbit secondary antibodies bind rabbit-derived primary antibodies with high specificity, commonly used in western blot, IHC, and ELISA detection systems.
Anti-Goat Secondary Antibodies
Anti-goat secondary antibodies target goat-derived primary antibodies, providing dependable signal detection in a variety of research and diagnostic assays.
Biotin-Conjugated Secondary Antibodies
Biotin-conjugated secondary antibodies pair with streptavidin-based detection systems to deliver signal amplification and increased sensitivity in ELISA and IHC protocols.