Plant Tissue Culture Media

Optimal growth and morphogenesis of tissues may differ for different plants according to their nutritional requirements. Additionally, tissues from different parts of plants may also have different requirements for satisfactory growth. Tissue culture media were first developed from nutrient solutions used for culturing whole plants, for example root culture medium of White and callus culture medium of Gautheret. White’s medium was based on Uspenski and Uspenska’s medium for algae, Gautheret’s medium was based on Knop’s salt solution. Basic media that are frequently used include Murashige and Skoog (MS) medium, Linsmaier and Skoog (LS) medium, Gamborg (B5) medium and Nitsch and Nitsch (NN) medium.