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Insect cell culture is a common choice for heterologous protein expression. For large-scale production or basic research, insect cells are able to express large quantities of protein with complex post-translational modifications. The Insect cell culture medium plays an essential role in providing an optimal environment for cell growth and maintenance. Commonly used media, such as Grace or Schneider, are used as a base. They are supplemented with fetal bovine serum (FBS) at concentrations of 5 to 10%, ensuring the supply of essential nutrients and growth factors crucial for cell vitality.

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